CRHR1 Polyklonaler Antikörper

CRHR1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 20967-1-AP

Synonyme

CRF R, CRF R 1, CRF R1, CRF1, CRF1R, CRFR, CRFR 1, CRFR1, CRH R 1, CRH R1, CRH R1h, CRHR, CRHR1, CRHR1f

---

• 

  WB analysis of HeLa using 20967-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20967-1-AP (CRHR1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20967-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20967-1-AP (CRHR1 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 4 publications below

Produktinformation

20967-1-AP bindet in WB, IF, ELISA CRHR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: corticotropin releasing hormone receptor 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 46-55 kDa
• GenBank-Zugangsnummer: NM_004382
• Gene symbol: CRHR1
• Gene ID (NCBI): 1394
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Corticotropin-releasing hormone (CRH, also known as Corticotropin-releasing factor, CRF) is a 41 amino acid peptide hormone and a key regulator of the stress response. CRH and the related urocortin peptides (UCN, UCN2 and UCN3) mediate their actions through two mammalian receptor subtypes, CRHR1 and CRHR2 (PMID: 23863939). CRHR1 (also known as CRFR1) exhibits high affinity towards CRH and UCN but low affinity towards UCN2 and no affinity towards UCN3 (PMID: 22684850). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Widely distributed throughout the brain, CRHR1 critically controls behavioral adaptation to stress and is causally linked to emotional disorders (PMID: 21885734). Alternative splicing results in multiple transcript variants encoding distinct isoforms of CRHR1. This antibody raised against a synthesized peptide corresponding to amino acid residues 148-165 of isoform CRF-R1 can only recognize isoform CRF-R1.

Publikationen

Species	Application	Title
	IF	Proc Natl Acad Sci U S A; Overactivation of corticotropin-releasing factor receptor type 1 and aquaporin-4 by hypoxia induces cerebral edema.; Authors - Shao-Jun Chen
human	WB,IF	Pharmaceuticals (Basel); A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors; Authors - Yueshan Sun
	WB	Behav Brain Res; Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.; Authors - Shuai Lian
	WB	Brain Res; Response of the maternal hypothalamus to cold stress during late pregnancy in rats.; Authors - Di Wang
	WB	Int J Mol Med; Sini San ameliorates duodenal mucosal barrier injury and low‑grade inflammation via the CRF pathway in a rat model of functional dyspepsia.; Authors - Chunyang Zhu
	WB	Reprod Toxicol; Dynamic expression of corticotropin-releasing hormone and urocortin in estrogen induced-cholestasis pregnant rat.; Authors - Fan Zhou