CRIP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CRIP2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 14801-1-AP

Synonyme

CRIP, CRIP 2, CRP 2, CRP2, CRP-2

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  WB analysis of mouse heart using 14801-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14801-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14801-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14801-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14801-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14801-1-AP

  Immunofluorescent analysis of HeLa cells using 14801-1-AP (CRIP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14801-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CRIP2 antibody (14801-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, Mauslungengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

14801-1-AP bindet in WB, IF/ICC, IP, ELISA CRIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRIP2 fusion protein Ag6515
• Vollständiger Name: cysteine-rich protein 2
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 23-29 kDa
• GenBank-Zugangsnummer: BC000434
• Gene symbol: CRIP2
• Gene ID (NCBI): 1397
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRIP2, also known as CRP2 and ESP1, belongs to the cysteine-rich intestinal protein family (CRIP family). The CRIP family is a subfamily of the highly conserved Lin-1, Isl1, Mec3/double zinc finger protein family that exhibits diverse biological functions. CRIP2 is a cardiac vascular marker, and its expression is detected in cardiac endothelial cells during development and in the adult heart(PMID: 40118853).

Publikationen

Species	Application	Title
rat	WB,IP,IF	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
zebrafish,human	WB,IF	Cell Mol Life Sci; Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation; Authors - Shuaiqi Yang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (06-07-2019)

The CRIP2 antibody works well and the protein shows up prominently as the lower band.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

CRIP2 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:14801-1-AP)