CRISP2 Polyklonaler Antikörper

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• KD/KO Validated

CRISP2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 19066-1-AP

Synonyme

Cancer/testis antigen 36, CRISP 2, CRISP2, CT36, GAPDL5, Testis specific protein TPX 1, TPX1, TSP1

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Galerie der Validierungsdaten

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  WB analysis using 19066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19066-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19066-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19066-1-AP (CRISP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19066-1-AP

  IP Result of anti-CRISP2 (IP:19066-1-AP, 4ug; Detection:19066-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 19066-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 19066-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19066-1-AP (CRISP2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, humanes Hodengewebe, Rattenhodengewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 2 publications below

Produktinformation

19066-1-AP bindet in WB, IP, IHC, IF, ELISA CRISP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRISP2 fusion protein Ag13574
• Vollständiger Name: cysteine-rich secretory protein 2
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC022011
• Gene symbol: CRISP2
• Gene ID (NCBI): 7180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice.; Authors - Deqiang Ding
mouse	WB	Nucleic Acids Res; DAZAP1 regulates the splicing of Crem, Crisp2 and Pot1a transcripts.; Authors - Chen Hsiang-Ying HY
mouse	WB	FASEB J; Functional redundancy and compensation: Deletion of multiple murine Crisp genes reveals their essential role for male fertility.; Authors - L Curci
human	WB	Biol Reprod; The Expression of Cysteine-Rich Secretory Protein 2 (CRISP2) and Its Specific Regulator miR-27b in the Spermatozoa of Patients with Asthenozoospermia.; Authors - Jun-Hao Zhou
mouse	WB	Mol Hum Reprod; Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders.; Authors - N G Brukman ; KO Validated
pig	IF,WB	Biol Reprod; Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa.; Authors - M Zhang