CRP Monoklonaler Antikörper

CRP Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

3F10A11

Kat-Nr. : 66250-1-Ig

Synonyme

C reactive protein, CRP, PTX1

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Galerie der Validierungsdaten

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  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours.

• 

  WB analysis of serum from mouse injected with bacteria using 66250-1-Ig

  Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 66250-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 66250-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (CRP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 66250-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (CRP Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of human liver using 66250-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Bakterien injiziertes Serum aus der Maus, Hausschwein-Lebergewebe, mit Bakteriengewebe injiziertes Serum aus der Maus, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Lebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66250-1-Ig bindet in WB, IHC, IF, ELISA CRP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRP fusion protein Ag9883
• Vollständiger Name: C-reactive protein, pentraxin-related
• Berechnetes Molekulargewicht: 224 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC020766
• Gene symbol: CRP
• Gene ID (NCBI): 1401
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Publikationen

Species	Application	Title
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
mouse	WB	Front Immunol; Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.; Authors - Anika Alberts
		Sci Rep; A label-free fiber optic SPR biosensor for specific detection of C-reactive protein.; Authors - Wenjia Wang
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
rat	WB,IHC	Brain Behav; Prenatal stress induced depressive-like behavior and region dependently high CRP level in offspring rats.; Authors - Shaoning Li
monkey	WB	Int J Radiat Oncol Biol Phys; Identification of Novel Biomarkers for Acute Radiation Injury Using Multiomics Approach and Nonhuman Primate Model; Authors - Amrita K Cheema

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