TORC1/CRTC1 Polyklonaler Antikörper

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• KD/KO Validated

TORC1/CRTC1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10441-1-AP

Synonyme

CRTC1, KIAA0616, MECT1, TORC 1, TORC1, TORC1/CRTC1, Transducer of CREB protein 1, WAMTP1

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 10441-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10441-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10441-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10441-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10441-1-AP

  IP Result of anti-TORC1 (IP:10441-1-AP, 4ug; Detection:10441-1-AP 1:800) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon cancer using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, A431-Zellen, Jurkat-Zellen, L02-Zellen, Maushirngewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

10441-1-AP bindet in WB, IP, IHC, IF, ELISA TORC1/CRTC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TORC1/CRTC1 fusion protein Ag0693
• Vollständiger Name: CREB regulated transcription coactivator 1
• Berechnetes Molekulargewicht: 75 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC028050
• Gene symbol: CRTC1
• Gene ID (NCBI): 23373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRTC1, also named as MECT1,TORC1, and WAMTP1, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. CRTC1 acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. It enhances the interaction of CREB1 with TAF4. CRTC1 regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. It is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. CRTC1 is also a coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, CRTC1 involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. It may be required for dendritic growth of developing cortical neurons. This is a rabbit polyclonal antibody raised against residues near N terminus of human CRTC1. CRTC1 has some isoforms produced by alternative splicing with MW of 67 kDa, 68 kDa and 62 kDa. Sometimes it appears as a 75-80 kDa band probably due to phosphorylation (PMID: 17183642; 22770221)

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Eosinophil extracellular traps drive asthma progression through neuro-immune signals.; Authors - Yiwen Lu ; KD Validated
mouse	WB,IP	iScience; Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction; Authors - Huanjuan Su
	IHC	J Ethnopharmacol; Exploring the potential mechanism of Heng-Gu-Gu-Shang-Yu-He-Ji therapy for osteoporosis based on network pharmacology and transcriptomics; Authors - Linbi Xie
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
		Ann N Y Acad Sci; PMID: 26645136; Authors - Holly M Brown-Borg
rat	WB,IF	J Agric Food Chem; Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction.; Authors - Guoyu Zhou