TORC1/CRTC1 Polyklonaler Antikörper

TORC1/CRTC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27647-1-AP

Synonyme

CRTC1, CREB-regulated transcription coactivator 1, KIAA0616, MECT1, TORC 1

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  WB analysis of Neuro-2a using 27647-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27647-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27647-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27647-1-AP (CRTC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 27647-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27647-1-AP (CRTC1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HEK-293T-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27647-1-AP bindet in WB, IHC, ELISA TORC1/CRTC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TORC1/CRTC1 fusion protein Ag26494
• Vollständiger Name: CREB regulated transcription coactivator 1
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 68-80 kDa
• GenBank-Zugangsnummer: BC017075
• Gene symbol: CRTC1
• Gene ID (NCBI): 23373
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRTC1, also named as Transducer of CREB protein 1, is a 634 amino acid protein, which belongs to the TORC family. CRTC1 is highly expressed in adult and fetal brain and is low expressed in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. CRTC1 localizes in Cytoplasm when is phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (PubMed:16817901). it translocates to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. CRTC1 as a Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. The calculated molecular weight of CRTC1 is 68 kDa, but the modified protein is about 70-75 kDa.

Publikationen

Species	Application	Title
human	WB	Autophagy; Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing.; Authors - Lei Qiang
rat	WB	Brain Res; The α1 adrenoceptor antagonist prazosin potentiates morphine induced conditioned place preference in rats; Authors - Wanyu Tu