Cryptochrome 2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cryptochrome 2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(39)

Rezensionen (2)

Kat-Nr. : 13997-1-AP

Synonyme

CRY 2, CRY2, Cryptochrome-2, HCRY2, PHLL2

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  WB analysis using 13997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13997-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13997-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13997-1-AP

  IP result of anti-Cryptochrome 2 (IP:13997-1-AP, 4ug; Detection:13997-1-AP 1:300) with mouse brain tissue lysate 5200ug.

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  IHC staining of mouse brain using 13997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13997-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 13997-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 13997-1-AP (Cryptochrome 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 37 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

13997-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Cryptochrome 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cryptochrome 2 fusion protein Ag5085
• Vollständiger Name: cryptochrome 2 (photolyase-like)
• Berechnetes Molekulargewicht: 593 aa, 67 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC041814
• Gene symbol: CRY2
• Gene ID (NCBI): 1408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cryptochrome circadian clock 2 (CRY2) is a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. Loss of CRY2 stabilizes c-MYC and enhances cellular transformation. CRY2 can function as a co-factor for the SCF substrate adaptor FBXL3 (PMID: 27840026). CRY2 has two isoform with MW of 60 and 67 kDa. This antibody can recognize CRY1 and CRY2 due to the high homology.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Time-of-day specificity of anticancer drugs may be mediated by circadian regulation of the cell cycle.; Authors - Yool Lee
rat	WB	Cell Death Differ; CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression.; Authors - Dandan Zhang
mouse	WB	Curr Biol; Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.; Authors - Luca Rizzini
human	WB	Elife; KPNB1 mediates PER/CRY nuclear translocation and circadian clock function.; Authors - Yool Lee
rat	WB	J Neuroimmune Pharmacol; Potential Role of Bmal1 in Lipopolysaccharide-Induced Depression-Like Behavior and its Associated "Inflammatory Storm"; Authors - Dan-Dan Xu
mouse,human	WB,IF	Mol Ther Nucleic Acids; Inhibition of CRY2 by STAT3/miRNA-7-5p Promotes Osteoblast Differentiation through Upregulation of CLOCK/BMAL1/P300 Expression.; Authors - Zhenghui Tang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Priya (Verified Customer) (01-11-2023)

I have used this for human cardiomyocytes cell line and mice liver and kin tisssue

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cardiomyoctyes, Keratinocytes, Liver and skin

FH

Julia (Verified Customer) (01-14-2022)

Gives one clear band with the expected size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: U2OS

Cryptochrome 2 Antibody Western Blot validation (1:800 dilution) in U2OS (Cat no:13997-1-AP)