CRYBB1 Monoklonaler Antikörper

CRYBB1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

6F12B12

Kat-Nr. : 60273-1-Ig

Synonyme

6F12B12, Beta B1 crystallin, Beta crystallin B1, Beta-B1 crystallin, Beta-crystallin B1

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  WB analysis of rabbit eye using 60273-1-Ig

  rabbit eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat eye using 60273-1-Ig

  rat eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 60273-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 60273-1-Ig

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 60273-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CRYBB1 antibody (60273-1-Ig, Clone: 6F12B12 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Augengewebe, C6-Zellen, Maus-Augengewebe
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60273-1-Ig bindet in WB, IF/ICC, ELISA CRYBB1 und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRYBB1 fusion protein Ag4722
• Vollständiger Name: crystallin, beta B1
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC036790
• Gene symbol: CRYBB1
• Gene ID (NCBI): 1414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.