CSNK1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CSNK1A1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 55192-1-AP

Synonyme

casein kinase 1, alpha 1, Casein kinase I isoform alpha, CK1, CKI alpha, CSNK1A1, HLCDGP1, PRO2975

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Galerie der Validierungsdaten

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  WB analysis using 55192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55192-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55192-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55192-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55192-1-AP (CSNK1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 55192-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of A549 cells using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 55192-1-AP

  Immunofluorescent analysis of A549 cells using 55192-1-AP (CSNK1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55192-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA CSNK1A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: casein kinase 1, alpha 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa, 42-45 kDa
• GenBank-Zugangsnummer: NM_001892
• Gene symbol: CSNK1A1
• Gene ID (NCBI): 1452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK1A1, also named as CK1, belongs to the protein kinase superfamily, CK1 Ser/Thr protein kinase family and Casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. CSNK1A1 phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis. This antibody is specific to CSNK1A1.

Publikationen

Species	Application	Title
human	WB,IHC	Pharmacol Res; Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex; Authors - Li Gu ; KD Validated
	WB	J Cell Biol; CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.; Authors - Xue Deng
human	IP	Virol Sin; CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells; Authors - Jing Xiong
mouse	WB,IF	Stem Cell Res Ther; Nicotinamide improves in vitro lens regeneration in a mouse capsular bag model.; Authors - Xiaomin Liu
human	WB	Int J Biol Sci; Pantothenate Kinase 1 Inhibits the Progression of Hepatocellular Carcinoma by Negatively Regulating Wnt/β-catenin Signaling.; Authors - Yuyuan Zi
mouse	WB,IF	Endocrinology; Casein kinase 1α regulates testosterone synthesis and testis development in adult mice; Authors - Hongzhou Guo