• Featured Product
  • KD/KO Validated

Casein Kinase 1 Delta Polyklonaler Antikörper

Casein Kinase 1 Delta Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(17)

Kat-Nr. : 14388-1-AP

Synonyme

EC:2.7.11.26, EC:2.7.11.1, CSNK1D, CKI-delta, CKId



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, K-562-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHCMaushirngewebe, Mausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCU-251-Zellen, HepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14388-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Casein Kinase 1 Delta und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Casein Kinase 1 Delta fusion protein Ag5856
Vollständiger Name casein kinase 1, delta
Berechnetes Molekulargewicht 47 kDa
Beobachtetes Molekulargewicht 47 kDa
GenBank-ZugangsnummerBC003558
Gene symbol CK1 Delta
Gene ID (NCBI) 1453
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK1D(casein kinase I isoform delta) is also named as HCKID, CKId and belongs to the casein kinase I subfamily. CKI is a ubiquitous enzyme which has been found in the cytosol, associated with membranes, and in the nucleus. It can phosphorylate a large number of cytosolic, membrane-associated, and nuclear proteins(PMID:8454611). CSNK1D is found to be associated generally with granulovacuolar bodies and tau-containing neurofibrillary tangles in AD, DS, PSP, PDC, PPND, and controls, and Pick bodies and ballooned neurons in PiD(PMID:10924763). It has 2 isoforms produced by alternative splicing.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Casein Kinase 1 Delta antibody 14388-1-APProtokoll herunterladen
IHC protocol for Casein Kinase 1 Delta antibody 14388-1-APProtokoll herunterladenl
IF protocol for Casein Kinase 1 Delta antibody 14388-1-APProtokoll herunterladen
IP protocol for Casein Kinase 1 Delta antibody 14388-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Mol Cancer

SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.

Authors - Qiwei Jiang
humanWB

Dev Cell

Mitotic ER Exit Site Disassembly and Reassembly Are Regulated by the Phosphorylation Status of TANGO1.

Authors - Miharu Maeda
humanWB

J Cell Biol

CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.

Authors - Xue Deng
  • KD Validated
humanWB

Cell Chem Biol

CDK2 heterobifunctional degraders co-degrade CDK2 and cyclin E resulting in efficacy in CCNE1-amplified and overexpressed cancers

Authors - Nicholas Kwiatkowski
humanWB

Oncogene

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

Authors - Yanwei Lu
  • KD Validated
humanWB

Mol Cell Biol

DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.

Authors - Chen Hao H

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Christina (Verified Customer) (11-28-2023)

I used the antibody to detect CKD1δ in WB after siRNA of CK1δ (MCF7 cells).

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MCF7
Casein Kinase 1 Delta Antibody Western Blot validation (1:2000 dilution) in MCF7 (Cat no:14388-1-AP)