CSNK2B Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CSNK2B Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B5A6

Kat-Nr. : 67866-1-Ig

Synonyme

Casein Kinase 2 beta, Casein kinase II subunit beta, CK II beta, CK2B, CK2N, CSK2B, CSNK2B, G5A, Phosvitin, Protein G5a

---

Galerie der Validierungsdaten

• 

  WB analysis using 67866-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67866-1-Ig (CSNK2B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67866-1-Ig

  WB result of CSNK2B antibody (67866-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CSNK2B transfected MCF-7 cells.

• 

  IHC staining of human breast cancer using 67866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 67866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67866-1-Ig (CSNK2B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67866-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CSNK2B antibody (67866-1-Ig, Clone: 1B5A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Hirngewebe, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67866-1-Ig bindet in WB, IHC, IF, ELISA CSNK2B und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CSNK2B fusion protein Ag19180
• Vollständiger Name: casein kinase 2, beta polypeptide
• Berechnetes Molekulargewicht: 215 aa, 25 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC112017
• Gene symbol: CSNK2B
• Gene ID (NCBI): 1460
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSNK2B is a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. It participates in Wnt signaling, and plays a complex role in regulating the basal catalytic activity of the alpha subunit.