CSRP1 Polyklonaler Antikörper

CSRP1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 13432-1-AP

Synonyme

CRP, CRP1, CSRP, CYRP, Cysteine and glycine-rich protein 1

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  WB analysis of human stomach using 13432-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 13432-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13432-1-AP (CSRP1 antibody) at dilution of 1:300.

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  IHC staining of human colon using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13432-1-AP (CSRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 13432-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13432-1-AP (CSRP1 Antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Magengewebe
• Erfolgreiche IP: Maus-Magengewebe
• Erfolgreiche Detektion in IHC: human normal colon, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

13432-1-AP bindet in WB, IHC, IF, IP, ELISA CSRP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSRP1 fusion protein Ag4236
• Vollständiger Name: cysteine and glycine-rich protein 1
• Berechnetes Molekulargewicht: 193 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC032493
• Gene symbol: CSRP1
• Gene ID (NCBI): 1465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSRP1 (Cysteine and glycine-rich protein 1), designated initially as CRP1, constitutes a cysteine-rich protein derived from placental sources and exhibits a response profile analogous to c-myc. CSRP1 has been recognized as a tumor suppressor in several types of cancers including colorectal cancer, and cholangiocarcinoma (PMID: 37113556). Abnormal expression of CSRP1 was reported within several malignancies such as prostate cancer and acute myeloid leukemia (PMID: 38813484, 40198006).

Publikationen

Species	Application	Title
mouse	WB,IF	Mol Psychiatry; PD-L1/PD-1 checkpoint pathway regulates astrocyte morphogenesis and myelination during brain development; Authors - Yanyan Wang
rat	WB	Mol Med Rep; Inflammatory and oxidative stress‑associated factors in chronic intermittent hypoxia in Chinese patients, rats, lymphocytes and endotheliocytes.; Authors - Hui Ren