CSRP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CSRP2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 10892-2-AP

Synonyme

CRP2, Cysteine and glycine-rich protein 2, Cysteine rich protein 2, Cysteine-rich protein 2, LMO5

---

• 

  WB analysis using 10892-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10892-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10892-2-AP (CSRP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 10892-2-AP

  IP result of anti-CSRP2 (IP:10892-2-AP, 4ug; Detection:10892-2-AP 1:1000) with mouse liver tissue lysate 4000ug.

• 

  IHC staining of mouse kidney using 10892-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10892-2-AP (CSRP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, Mausnierengewebe, Rattennierengewebe, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10892-2-AP bindet in WB, IHC, IF, IP, CoIP, ELISA CSRP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSRP2 fusion protein Ag1338
• Vollständiger Name: cysteine and glycine-rich protein 2
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC000992
• Gene symbol: CSRP2
• Gene ID (NCBI): 1466
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSRP2 (Cysteine and glycine-rich protein 2) is a member of the LIM-only family of cysteine-rich proteins and contains two LIM domains, which has been recently implicated in the progression and metastasis of a variety of cancers. CSRP2 is functionally linked to the activity of TGF-b1 in hepatic stellate cells (HSC), derivatives thereof, and in differentiated VSMC.CSRP2 knockdown signifcantly inhibits hypoxia-stimulated invadopodium formation, ECM degradation and invasion in MDA-MB-231 cells (PMID: 33042270). CSRP2 is a short (21 kDa) two LIM domain-containing protein, which is upregulated in invasive breast cancer cells, and localizes along the protrusive actin core of invadopodium1 (PMID: 29976963, 16735029).

Publikationen

Species	Application	Title
human	WB	Sci Rep; Proteotoxicity in cardiac amyloidosis: amyloidogenic light chains affect the levels of intracellular proteins in human heart cells.; Authors - Esther Imperlini
rat	WB,IF	Toxicol Appl Pharmacol; Cysteine and glycine rich protein 2 exacerbates vascular fibrosis in pulmonary hypertension through the nuclear translocation of yes-associated protein and transcriptional coactivator with PDZ-binding motif; Authors - Xinghe Chen ; KD Validated
human	WB,IHC	J Med Dent Sci; TDP-43 in the skin of amyotrophic lateral sclerosis patients.; Authors - Keisuke Abe
mouse	WB	Cell Biochem Funct; Cysteine and glycine-rich protein 2 retards platelet-derived growth factor-BB-evoked phenotypic transition of airway smooth muscle cells by decreasing YAP/TAZ activity; Authors - Huiyuan Wang ; KD Validated
mouse,human	WB,IHC	Toxicol Appl Pharmacol; Cysteine and glycine-rich protein 2 is crucial for maintaining the malignant phenotypes of gliomas through its action on Notch signalling cascade; Authors - Lingtong Liu
human	WB,IHC,CoIP	Oncol Rep; Cysteine‑ and glycine‑rich protein 2: A vital regulator that inhibits necroptosis glioma cell by activating the JAK‑STAT1 pathways; Authors - Dongsheng Lv ; KD Validated