CSRP3 Polyklonaler Antikörper

CSRP3 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 10721-1-AP

Synonyme

Cardiac LIM protein, CMD1M, CRP3, Cysteine and glycine-rich protein 3, Cysteine rich protein 3

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  WB analysis of mouse heart using 10721-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10721-1-AP (CSRP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10721-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10721-1-AP (CSRP3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10721-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10721-1-AP (CSRP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 10721-1-AP

  IP result of anti-CSRP3 (IP:10721-1-AP, 4ug; Detection:10721-1-AP 1:2000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 10721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10721-1-AP (CSRP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 10721-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10721-1-AP (CSRP3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10721-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10721-1-AP (CSRP3 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

10721-1-AP bindet in WB, IHC, IP, ELISA CSRP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CSRP3 fusion protein Ag1136
• Vollständiger Name: cysteine and glycine-rich protein 3 (cardiac LIM protein)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005900
• Gene symbol: CSRP3
• Gene ID (NCBI): 8048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. The cytoplasmic CSRP3 serves as a scaffold protein that primarily interacts and co-localizes with different Z-disc components of myofibrils (PMID: 31979369, 17519359).

Publikationen

Species	Application	Title
mouse	WB	FASEB J; Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.; Authors - Julia Straubinger
mouse	WB	Hum Mol Genet; Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.; Authors - Aymeric Ravel-Chapuis
human	IHC	J Gene Med; CSRP3, p.Arg122*, is Responsible for Hypertrophic Cardiomyopathy in a Chinese Family.; Authors - Hao Huang
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (03-03-2022)

Very strong single band by comparing the KO tissues

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: Mouse Heart