CST1 Polyklonaler Antikörper

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• KD/KO Validated

CST1 Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 16025-1-AP

Synonyme

CST1, Cystain SA I, Cystatin 1, cystatin SN, Salivary cystatin SA 1

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Galerie der Validierungsdaten

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  WB analysis of human saliva using 16025-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 16025-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 16025-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16025-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 16025-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CST1 (16025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Speichelgewebe, COLO 320-Zellen, humanes Hodengewebe, humanes Nebennierengewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16025-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA CST1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CST1 fusion protein Ag8862
• Vollständiger Name: cystatin SN
• Berechnetes Molekulargewicht: 141 aa, 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa
• GenBank-Zugangsnummer: BC021225
• Gene symbol: CST1
• Gene ID (NCBI): 1469
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
human	IHC,IF	Nat Commun; Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment.; Authors - Huy Q Dinh
human	WB	Cell Res; Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.; Authors - Jin Lei L
human	WB,IHC,IF,IP	Oncogene; CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1; Authors - Dongbao Li ; KD Validated
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	IHC	Aging (Albany NY); Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.; Authors - Jing Han