CTGF Polyklonaler Antikörper

CTGF Polyklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 25474-1-AP

Synonyme

CCN family member 2, CCN2, CTGF, HCS24, IBP 8, IGF binding protein 8, IGFBP 8, IGFBP8, NOV2

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Galerie der Validierungsdaten

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  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25474-1-AP (CTGF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HuH-7 using 25474-1-AP

  1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human CTGF (25474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in FC: HuH-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

25474-1-AP bindet in WB, IHC, IF, FC, ELISA CTGF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: connective tissue growth factor
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_001901
• Gene symbol: CTGF
• Gene ID (NCBI): 1490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTGF, also known as CCN2, is a member of the CCN family of matricellular proteins. CTGF, a cysteine-rich, matrix-associated, heparin-binding protein, is widely expressed in various human tissues and organs. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo.; Authors - Wen-Chih Liu
pig	WB	Front Bioeng Biotechnol; Effect of stem cell conditional medium-loading adhesive hydrogel on TGF-β1-induced endometrial stromal cell fibrosis; Authors - Yuan Zhu
rat	WB	Chin Med; Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis.; Authors - Wenchuan Qi
mouse	WB,IF	J Agric Food Chem; Krill Oil Turns Off TGF-β1 Profibrotic Signaling in the Prevention of Diabetic Nephropathy; Authors - Xuechun Sun
human,mouse	WB,IHC	Front Pharmacol; 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways; Authors - Tsung-Teng Huang
mouse	WB	Life Sci; Verteporfin inhibits the dedifferentiation of tubular epithelial cells via TGF-β1/Smad pathway but induces podocyte loss in diabetic nephropathy; Authors - Chenyang Qi