Alpha E-Catenin Polyklonaler Antikörper

Alpha E-Catenin Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 12831-1-AP

Synonyme

alpha catenin, Alpha E catenin, Alpha E-Catenin, Cadherin associated protein, CAP102, Catenin alpha 1, CTNNA1, α-catenin, α-E-catenin, αE-catenin

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Galerie der Validierungsdaten

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  WB analysis of mouse uterus using 12831-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12831-1-AP (Alpha E catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12831-1-AP

  IP Result of anti-Alpha E catenin (IP:12831-1-AP, 4ug; Detection:12831-1-AP 1:700) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 12831-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12831-1-AP (Alpha E catenin antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 12831-1-AP

  Immunofluorescent analysis of A431 cells using 12831-1-AP (Alpha E catenin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of MCF-7 using 12831-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Alpha E catenin antibody (12831-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Uterusgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Kolongewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IF: See 2 publications below

Produktinformation

12831-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Alpha E-Catenin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Alpha E-Catenin fusion protein Ag3565
• Vollständiger Name: catenin (cadherin-associated protein), alpha 1, 102kDa
• Berechnetes Molekulargewicht: 906 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC031262
• Gene symbol: CTNNA1
• Gene ID (NCBI): 1495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. It also recruits a range of other important proteins to developing intercellular junctions. Three alpha catenins exist in human: alpha-E-catenin, alpha-N-catenin, and alpha-T-catenin, which share substantial amino-acid sequence similarity but have distinct tissue distribution. alpha-E-catenin is ubiquitously expressed, alpha-N-catenin is restricted to neuronal tissue, and alpha-T-catenin is primarily expressed in heart tissue. Reduced levels of alpha-E-catenin protein seem to be characteristic of many different human cancers, including malignant tumours of the breast, colon, stomach, oesophagus, bladder and liver. In addition, the loss of alpha-E-catenin often correlates with the degree of tumour differentiation and metastasis.

Publikationen

Species	Application	Title
human	WB	Oncogene; Transducin (β)-like 1 X-linked receptor 1 promotes gastric cancer progression via the ERK1/2 pathway.; Authors - Q Zhou
mouse	IF	Elife; Selective YAP activation in Procr cells is essential for ovarian stem/progenitor expansion and epithelium repair.; Authors - Jingqiang Wang
human	WB	Oncogene; The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.; Authors - Quan Zhou
human	WB	Helicobacter; Helicobacter pylori Dwelling on the Apical Surface of Gastrointestinal Epithelium Damages the Mucosal Barrier Through Direct Contact.; Authors - Chen Zhang
human	WB	Cells; P-Cadherin Regulates Intestinal Epithelial Cell Migration and Mucosal Repair, but Is Dispensable for Colitis Associated Colon Cancer.; Authors - Nayden G Naydenov
human	IF	Sci Rep; SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth.; Authors - Rehab Alshargabi

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