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  • KD/KO Validated

Beta Catenin Monoklonaler Antikörper

Beta Catenin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(121)

CloneNo.

1B8A1

Kat-Nr. : 66379-1-Ig

Synonyme

Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA431-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe
Erfolgreiche IPMaushirngewebe
Erfolgreiche Detektion in IHCMaus-Kolongewebe, humanes Mammakarzinomgewebe, humanes Kolongewebe, humanes Hautkrebsgewebe, Ratten-Kolongewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Kolonkarzinomgewebe, humanes Hautkrebsgewebe
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen, humanes Leberkarzinomgewebe, humanes Hautkrebsgewebe
Erfolgreiche Detektion in FC (Intra)MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:10000-1:40000
Immunfluoreszenz (IF)-PIF-P : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:750-1:3000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66379-1-Ig bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA Beta Catenin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Huhn, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name catenin (cadherin-associated protein), beta 1, 88kDa
Berechnetes Molekulargewicht 781 aa, 86 kDa
Beobachtetes Molekulargewicht 92 kDa
GenBank-ZugangsnummerNM_001904
Gene symbol Beta Catenin
Gene ID (NCBI) 1499
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Beta Catenin antibody 66379-1-IgProtokoll herunterladen
IHC protocol for Beta Catenin antibody 66379-1-IgProtokoll herunterladenl
IF protocol for Beta Catenin antibody 66379-1-IgProtokoll herunterladen
IP protocol for Beta Catenin antibody 66379-1-IgProtokoll herunterladen
FC protocol for Beta Catenin antibody 66379-1-IgDownload protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Adv Mater

Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment

Authors - Yuewen Zhai
mouse,ratWB

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
humanWB

J Exp Clin Cancer Res

Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma

Authors - Yongzhi Zhao
  • KD Validated
humanIF

Research (Wash D C)

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling

Authors - Xi Chen
humanIF

Acta Biomater

Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering

Authors - Zhen Liu
mouseIF

Redox Biol

Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis.

Authors - Tirthankar Sinha

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Bruna (Verified Customer) (05-30-2024)

Great antibody! In our lab, it was used for Western blot and immunofluorescence detection. In both cases, the results were excellent.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Cancer- associated fibroblasts.