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Beta Catenin Monoklonaler Antikörper
Beta Catenin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
1B8A1
Kat-Nr. : 66379-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | A431-Zellen, HEK-293-Zellen, HeLa-Zellen, LNCaP-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe | 
| Erfolgreiche IP | Maushirngewebe | 
| Erfolgreiche Detektion in IHC | Maus-Kolongewebe,  humanes Mammakarzinomgewebe,  humanes Kolongewebe,  humanes Hautkrebsgewebe,  Ratten-Kolongewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. | 
| Erfolgreiche Detektion in IF-P | humanes Kolonkarzinomgewebe, humanes Hautkrebsgewebe | 
| Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen, humanes Leberkarzinomgewebe, humanes Hautkrebsgewebe | 
| Erfolgreiche Detektion in FC (Intra) | MCF-7-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunhistochemie (IHC) | IHC : 1:10000-1:40000 | 
| Immunfluoreszenz (IF)-P | IF-P : 1:200-1:800 | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:750-1:3000 | 
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 4 publications below | 
| WB | See 101 publications below | 
| IHC | See 12 publications below | 
| IF | See 36 publications below | 
| IP | See 1 publications below | 
| CoIP | See 7 publications below | 
Produktinformation
66379-1-Ig bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA Beta Catenin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte | 
| In Publikationen genannte Reaktivität | human, Hausschwein, Huhn, Maus, Ratte | 
| Wirt / Isotyp | Maus / IgG1 | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | Peptid | 
| Vollständiger Name | catenin (cadherin-associated protein), beta 1, 88kDa | 
| Berechnetes Molekulargewicht | 781 aa, 86 kDa | 
| Beobachtetes Molekulargewicht | 92 kDa | 
| GenBank-Zugangsnummer | NM_001904 | 
| Gene symbol | Beta Catenin | 
| Gene ID (NCBI) | 1499 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-G-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for Beta Catenin antibody 66379-1-Ig | Protokoll herunterladen | 
| IHC protocol for Beta Catenin antibody 66379-1-Ig | Protokoll herunterladenl | 
| IF protocol for Beta Catenin antibody 66379-1-Ig | Protokoll herunterladen | 
| IP protocol for Beta Catenin antibody 66379-1-Ig | Protokoll herunterladen | 
| FC protocol for Beta Catenin antibody 66379-1-Ig | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Publikationen
| Species | Application | Title | 
|---|---|---|
| Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
| Acta Pharm Sin B Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2. | ||
| J Exp Clin Cancer Res Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma
 | ||
| Research (Wash D C) The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling | ||
| Acta Biomater Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering | ||
| Redox Biol Riboflavin deficiency leads to irreversible cellular changes in the RPE and disrupts retinal function through alterations in cellular metabolic homeostasis. | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Bruna (Verified Customer) (05-30-2024) | Great antibody! In our lab, it was used for Western blot and immunofluorescence detection. In both cases, the results were excellent. 
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