CTP synthase Polyklonaler Antikörper

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• KD/KO Validated

CTP synthase Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 15914-1-AP

Synonyme

CTP synthase, CTP synthase 1, CTP synthetase 1, CTPS, CTPS 1, CTPS1, UTP ammonia ligase 1

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Galerie der Validierungsdaten

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  WB analysis using 15914-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15914-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15914-1-AP (CTP synthase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15914-1-AP

  IP Result of anti-CTP synthase (IP:15914-1-AP, 4ug; Detection:15914-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15914-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15914-1-AP (CTP synthase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15914-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTPS antibody 15914-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 15914-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CTP synthase antibody (15914-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Raji-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• CoIP: See 1 publications below

Produktinformation

15914-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA CTP synthase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Stechmücke, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTP synthase fusion protein Ag8707
• Vollständiger Name: CTP synthase
• Berechnetes Molekulargewicht: 591 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC009408
• Gene symbol: CTPS
• Gene ID (NCBI): 1503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTP synthase(CTPS) is also named as CTPS1 and belongs to the CTP synthase family. It catalyses the ATP-dependent formation of CTP from UTP using either L-glutamine or NH3 as the nitrogen source(PMID:12752439). It is important in the biosynthesis of phospholipids and nucleic acids, and plays a key role in cell growth, development, and tumorigenesis (PMID:8813694). CTP synthetase exists as a dimer in the absence of its substrates ATP and UTP, but in the presence of saturating concentrations of these substrates the enzyme exists as a tetramer.(PMID:18439916)

Publikationen

Species	Application	Title
human	WB	Cancer Res; Combined inactivation of CTPS1 and ATR is synthetically lethal to MYC-overexpressing cancer cells.; Authors - Zhe Sun
human	IF	FEBS J; Inter-filament interaction between IMPDH and CTPS cytoophidia.; Authors - Chia-Chun Chang
human	WB,CoIP	J Cell Physiol; Energy deficiency caused by CTPS downregulation in decidua may contribute to pre-eclampsia by impairing decidualization.; Authors - Ke Ma
human		Front Immunol; Flow Cytometry Contributions for the Diagnosis and Immunopathological Characterization of Primary Immunodeficiency Diseases With Immune Dysregulation.; Authors - Otavio Cabral-Marques
human	WB	Oncotarget; Identification and validation of differentially expressed proteins in epithelial ovarian cancers using quantitative proteomics.; Authors - Hong Qu
human	IF	J Cell Sci; Rod and Ring formation from IMP dehydrogenase is regulated via the one-carbon metabolic pathway.; Authors - S John Calise