CTR9 Polyklonaler Antikörper

CTR9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 21264-1-AP

Synonyme

CTR9, KIAA0155, p150, p150TSP, SH2 domain binding protein 1, SH2BP1, TSBP

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  WB analysis of Raji using 21264-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21264-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21264-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21264-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21264-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 21264-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21264-1-AP (CTR9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon cancer using 21264-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21264-1-AP (CTR9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 21264-1-AP

  Immunofluorescent analysis of Hela cells, using CTR9 antibody 21264-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HEK-293-Zellen, HeLa-Zellen, L02-Zellen, Mauslungengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

21264-1-AP bindet in WB, IHC, IF/ICC, ELISA CTR9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTR9 fusion protein Ag15690
• Vollständiger Name: Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 1173 aa, 134 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: BC058914
• Gene symbol: CTR9
• Gene ID (NCBI): 9646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Autophagy; The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.; Authors - Liangde Zheng
mouse	WB	Front Pharmacol; Tetrandrine Prevents Bone Loss in Ovariectomized Mice by Inhibiting RANKL-Induced Osteoclastogenesis.; Authors - Zeyuan Zhong
mouse	WB	Cell Death Dis; UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.; Authors - Gang Xiang
human	WB	Cell; SARS-CoV-2 variants evolve convergent strategies to remodel the host response; Authors - Mehdi Bouhaddou
human	IHC	Eur J Histochem; MicroRNA-17-3p is upregulated in psoriasis and regulates keratinocyte hyperproliferation and pro-inflammatory cytokine secretion by targeting CTR9; Authors - Qingwen Li