CUL4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CUL4B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

72

Kat-Nr. : 12916-1-AP

Synonyme

CUL 4B, CUL4B, cullin 4B, KIAA0695, MRXHF2, MRXSC, SFM2

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Galerie der Validierungsdaten

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  WB analysis using 12916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12916-1-AP (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12916-1-AP

  WB result of CUL4B antibody (12916-1-AP, 1:2,000) with si-Control and si-CUL4B transfected HeLa cells.

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  IP experiment of HeLa using 12916-1-AP

  IP Result of anti-CUL4B (IP:12916-1-AP, 3ug; Detection:12916-1-AP 1:1000) with HeLa cells lysate 4650ug.

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  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 12916-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (12916-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 27 publications below
• WB: See 67 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

12916-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA CUL4B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL4B fusion protein Ag3563
• Vollständiger Name: cullin 4B
• Berechnetes Molekulargewicht: 913 aa, 104 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC036216
• Gene symbol: CUL4B
• Gene ID (NCBI): 8450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Publikationen

Species	Application	Title
human	CoIP,WB	Cell; FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.; Authors - Jia Z Shen ; KD Validated
human	WB	Cell; Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.; Authors - Xiulei Mo
human	IHC,IF	Science; CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.; Authors - Chao Yu
human	WB	Nat Med; Immunomodulatory drugs disrupt the cereblon-CD147-MCT1 axis to exert antitumor activity and teratogenicity.; Authors - Ruth Eichner ; KD Validated
human	WB	Science; Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.; Authors - Ting Han
mouse	WB	Cancer Cell; Nuclear cyclin D1/CDK4 kinase regulates CUL4 expression and triggers neoplastic growth via activation of the PRMT5 methyltransferase.; Authors - Aggarwal Priya P

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