CUL4B Monoklonaler Antikörper

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• KD/KO Validated

CUL4B Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

4E10B10

Kat-Nr. : 60151-1-Ig

Synonyme

4E10B10, cullin 4B, Cullin-4B, KIAA0695

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  WB analysis using 60151-1-Ig

  various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 60151-1-Ig

  WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells.

• 

  WB analysis of HeLa using 60151-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60151-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60151-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 60151-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 60151-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, ROS1728-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

60151-1-Ig bindet in WB, IHC, ELISA CUL4B und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CUL4B fusion protein Ag3563
• Vollständiger Name: cullin 4B
• Berechnetes Molekulargewicht: 913 aa, 104 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC036216
• Gene symbol: CUL4B
• Gene ID (NCBI): 8450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).

Publikationen

Species	Application	Title
human		Curr Biol; Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex.; Authors - Masashi Minamino
		Int J Oncol; miR‑381 and miR‑489 suppress cell proliferation and invasion by targeting CUL4B via the Wnt/β‑catenin pathway in gastric cancer.; Authors - Ziling Fang
mouse	WB	Biochem Biophys Res Commun; Insulin alleviates LPS-induced ARDS via inhibiting CUL4B-mediated proteasomal degradation and restoring expression level of Na,K-ATPase α1 subunit through elevating HCF-1.; Authors - Xue-Ting Huang
human	WB,IHC	Pathol Res Pract; Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer.; Authors - Min Zhong
human	WB	Sci Adv; NEDDylated Cullin 3 mediates the adaptive response to topoisomerase 1 inhibitors; Authors - Alice Meroni ; KD Validated