CWC15 Polyklonaler Antikörper

CWC15 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25293-1-AP

Synonyme

Protein CWC15 homolog, ORF5, HSPC148, Cwf15, C11orf5

---

• 

  WB analysis of mouse liver using 25293-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 25293-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25293-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25293-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25293-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 25293-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 25293-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 25293-1-AP (CWC15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25293-1-AP

  IP result of anti-CWC15 (IP:25293-1-AP, 3ug; Detection:25293-1-AP 1:600) with HeLa cells lysate 2800ug.

• 

  IHC staining of human stomach using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25293-1-AP (CWC15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 25293-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25293-1-AP (CWC15 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 25293-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 25293-1-AP (CWC15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausherzgewebe, Maushodengewebe, PC-3-Zellen, SW 1990-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

25293-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CWC15 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CWC15 fusion protein Ag18116
• Vollständiger Name: CWC15 spliceosome-associated protein homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 229 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27-35 kDa
• GenBank-Zugangsnummer: BC040946
• Gene symbol: CWC15
• Gene ID (NCBI): 51503
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.

Publikationen

Species	Application	Title
	WB	Nucleic Acids Res; CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex.; Authors - Febe van Maldegem