CX3CR1 Monoklonaler Antikörper

CX3CR1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3D3B8

Kat-Nr. : 66915-1-Ig

Synonyme

Beta chemokine receptor like 1, Beta chemokine receptor-like 1, C X3 C CKR 1, CCRL1, CMK BRL 1

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  WB analysis using 66915-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66915-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66915-1-Ig (CX3CR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 66915-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66915-1-Ig (CX3CR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 66915-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CX3CR1 antibody (66915-1-Ig, Clone: 3D3B8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, SH-SY5Y-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66915-1-Ig bindet in WB, IHC, IF-P, ELISA CX3CR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CX3CR1 fusion protein Ag27698
• Vollständiger Name: chemokine (C-X3-C motif) receptor 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC028078
• Gene symbol: CX3CR1
• Gene ID (NCBI): 1524
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CX3CR1, also known as GPR13, V28, and fractalkine receptor, belongs to the 7-transmembrane G protein-coupled receptor (GPCR) family. It is expressed in microglia, astrocytes, NK cells, monocytes/macrophages, and a subpopulation of T cells. CX3CR1 is the receptor for fractalkine (CX3CL1) and mediates both its adhesive and migratory functions. It also acts as a coreceptor with CD4 for HIV-1 virus envelope protein (in vitro), and some variations in the gene of CX3CR1 lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Defects in CX3CR1 are a cause of susceptibility to age-related macular degeneration type 12 (ARMD12).

Publikationen

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Artificial Microglia Nanoplatform Loaded With Anti-RGMa in Acoustic/Magnetic Feld for Recanalization and Neuroprotection in Acute Ischemic Stroke; Authors - Ruiqi Cheng