CXCL10/IP10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CXCL10/IP10 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (1)

Kat-Nr. : 10937-1-AP

Synonyme

C X C motif chemokine 10, C7, crg 2, CXCL10, CXCL10/IP10, Gamma IP10, gIP 10, IFI10, INP10, IP 10, IP10, mob 1, SCYB10, Small inducible cytokine B10

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Galerie der Validierungsdaten

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  IHC staining of human ovary tumor using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nasopharyngeal carcinoma using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human nasopharyngeal carcinoma using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human nasopharyngeal carcinoma tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10937-1-AP (CXCL10/IP10 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10937-1-AP (IP10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10937-1-AP (IP10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Magenkrebsgewebe, humanes Nasopharynxkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 16 publications below
• IF: See 2 publications below

Produktinformation

10937-1-AP bindet in WB, IHC, IF, ELISA CXCL10/IP10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL10/IP10 fusion protein Ag1369
• Vollständiger Name: chemokine (C-X-C motif) ligand 10
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC010954
• Gene symbol: CXCL10
• Gene ID (NCBI): 3627
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.

Publikationen

Species	Application	Title
mouse	WB	Adv Healthc Mater; A Novel Tri-functional Liposome Re-educates "Cold Tumor" and Abrogates Tumor Growth by Synergizing Autophagy Inhibition and PD-L1 Blockade; Authors - Fei Zhou
human	IHC	J Mater Chem B; Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating; Authors - Bin Yan
human,mouse	IHC	Front Immunol; LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma; Authors - Houhong Wang
human	IHC	Cancer Commun (Lond); KRAS-G12D mutation drives immune suppression and the primary resistance of anti-PD-1/PD-L1 immunotherapy in non-small cell lung cancer.; Authors - Chengming Liu
human	IHC	Am J Cancer Res; Identification of potential immune-related circRNA-miRNA-mRNA regulatory network in cutaneous squamous cell carcinoma; Authors - Yuanfeng Wei
human	WB	Front Immunol; CD11c+ B Cells Participate in the Pathogenesis of Graves' Disease by Secreting Thyroid Autoantibodies and Cytokines.; Authors - Yedi Cao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Federica (Verified Customer) (11-07-2023)

I used Alkaline Phospatase Dako kit and Antigen Retrival PH10

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Healthy Lymphonode

CXCL10/IP10 Antibody Immunohistochemistry validation (1:200 dilution) in Healthy Lymphonode (Cat no:10937-1-AP)