CXCL12/SDF-1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CXCL12/SDF-1 Polyklonal Antikörper für FC, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

41

Rezensionen (1)

Kat-Nr. : 17402-1-AP

Synonyme

C X C motif chemokine 12, CXCL12, CXCL12/SDF-1, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF-1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1

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Galerie der Validierungsdaten

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  IHC staining of human liver cancer using 17402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17402-1-AP (CXCL12/SDF-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17402-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17402-1-AP (CXCL12/SDF-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of Jurkat using 17402-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human CXCL12/SDF-1 (17402-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 12 publications below
• IF: See 15 publications below

Produktinformation

17402-1-AP bindet in WB, IHC, IF, FC, ELISA CXCL12/SDF-1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL12/SDF-1 fusion protein Ag11379
• Vollständiger Name: chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
• Berechnetes Molekulargewicht: 89 aa, 10 kDa
• GenBank-Zugangsnummer: BC039893
• Gene symbol: CXCL12
• Gene ID (NCBI): 6387
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.

Publikationen

Species	Application	Title
	IF	Nature; Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis.; Authors - Bernardo Tavora
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
mouse	WB	Biomed Pharmacother; Angelica sinensis polysaccharides ameliorated 5-Fluorouracil-induced damage of early B cell progenitors by alleviating oxidative stress of IL-7 producing mesenchymal stem and progenitor cells; Authors - Hanxianzhi Xiao
human		Mol Oncol; Growth arrest-specific protein 2 (GAS2) interacts with CXCR4 to promote T-cell leukemogenesis partially via c-MYC; Authors - Yun Zhao
human	WB	J Nanobiotechnology; Magnetic targeting enhances the cutaneous wound healing effects of human mesenchymal stem cell-derived iron oxide exosomes.; Authors - Xiuying Li
human	WB	Fertil Steril; Protein kinase CK2 participates in estrogen-mediated endothelial progenitor cell homing to endometriotic lesions through stromal cells in a stromal cell-derived factor-1- CXCR4-dependent manner.; Authors - Rong Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yohanes (Verified Customer) (09-16-2022)

Good specificity antibody for immunofluoresence experiment.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse brain endothelial cell

CXCL12/SDF-1 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Mouse brain endothelial cell (Cat no:17402-1-AP)

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