CXCL2 Rekombinanter Antikörper

CXCL2 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

242007G5

Kat-Nr. : CL594-98259

Synonyme

242007G5, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Mip-2

---

• 

  FC experiment of RAW 264.7 using CL594-98259

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse CXCL2 Rabbit RecAb (CL594-98259, Clone: 242007G5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of RAW 264.7 using CL594-98259

  1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse CXCL2 Rabbit RecAb (CL594-98259, Clone: 242007G5) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug CoraLite®594 Anti-Mouse CXCL2 Rabbit RecAb (CL594-98259, Clone: 242007G5) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): mit LPS und Brefeldin A behandelte RAW 264.7-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98259 bindet in FC (Intra) CXCL2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL2 fusion protein Eg2239
• Vollständiger Name: chemokine (C-X-C motif) ligand 2
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_009140
• Gene symbol: Cxcl2
• Gene ID (NCBI): 20310
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).