CXCL7/PPBP Rekombinanter Antikörper

CXCL7/PPBP Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

250049B8

Kat-Nr. : 98497-3-RR

Synonyme

Chemokine (C-X-C motif) ligand 7, Cxcl7, Ppbp

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  FC experiment of Transfected HEK-293T using 98497-3-RR

  1x10^6 HEK-293T cells or CXCL7/PPBP transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat CXCL7/PPBP Rabbit RecAb (98497-3-RR, Clone: 250049B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98497-3-RR

  1x10^6 HEK-293T cells or CXCL7/PPBP transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat CXCL7/PPBP Rabbit RecAb (98497-3-RR, Clone: 250049B8) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98497-3-RR bindet in FC (Intra) CXCL7/PPBP und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL7/PPBP fusion protein Eg3405
• Vollständiger Name: pro-platelet basic protein (chemokine (C-X-C motif) ligand 7)
• Berechnetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: NM_153721.1
• Gene symbol: Ppbp
• Gene ID (NCBI): 246358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.