CXCL9/MIG Rekombinanter Antikörper

CXCL9/MIG Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

241437F5

Kat-Nr. : 98211-1-RR

Synonyme

MIG; CXCL9, MIG, CXCL9, C-X-C motif chemokine 9, CMK

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  FC experiment of human PBMCs using 98211-1-RR

  1x10^6 untreated or IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98211-1-RR

  1x10^6 IFN gamma, TNF α and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL9/MIG Rabbit Recombinant Antibody (98211-1-RR, Clone: 241437F5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): IFN gamma, TNF α and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98211-1-RR bindet in FC (Intra) CXCL9/MIG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL9/MIG fusion protein Ag17932
• Vollständiger Name: chemokine (C-X-C motif) ligand 9
• Berechnetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC095396
• Gene symbol: CXCL9
• Gene ID (NCBI): 4283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CXCL9 is a small cytokine known as MIG. CXCL9 is a T-cell chemoattractant, which is induced by IFN-γ. It is closely related to two other CXC chemokines called CXCL10 and CXCL11, whose genes are located near the gene for CXCL9 on human chromosome 4. A high level of CXCL9 revealed in peripheral liquids, can be considered as a marker of host immune response, especially of that involving Th1 cells.