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CXCR7 Monoklonaler Antikörper

CXCR7 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

4C3D7

Kat-Nr. : 60216-1-Ig

Synonyme

ACKR3, Chemokine orphan receptor 1, CMKOR1, CXC R7, CXCR 7, CXCR7, G protein coupled receptor 159, GPR159, RDC 1, RDC1

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CXCR7 Monoclonal antibody (60216-1-Ig)

    WB analysis using 60216-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60216-1-Ig (CXCR7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IHC staining of human breast cancer using 60216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IHC staining of human breast cancer using 60216-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60216-1-Ig (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human prostate cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human prostate cancer using 60216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human breast cancer using 60216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using CXCR7 Monoclonal antibody (60216-1-Ig)

    IF Staining of human breast cancer using 60216-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CXCR7 antibody (60216-1-Ig, Clone: 4C3D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, THP-1-Zellen, U-87 MG-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFhumanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 1 publications below

Produktinformation

60216-1-Ig bindet in WB, IHC, IF, ELISA CXCR7 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen CXCR7 fusion protein Ag14247
Vollständiger Name chemokine (C-X-C motif) receptor 7
Berechnetes Molekulargewicht 362 aa, 41 kDa
Beobachtetes Molekulargewicht 50-60 kDa
GenBank-ZugangsnummerBC036661
Gene symbol CXCR7
Gene ID (NCBI) 57007
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).

Protokolle

Produktspezifische Protokolle
WB protocol for CXCR7 antibody 60216-1-IgProtokoll herunterladen
IHC protocol for CXCR7 antibody 60216-1-IgProtokoll herunterladen
IF protocol for CXCR7 antibody 60216-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Urol Oncol

The inflammation-related biomarker CXCR7 independently predicts patient outcome after radical prostatectomy.

Authors - Pedro Bargão Santos
View Article