CXorf56 Polyklonaler Antikörper

CXorf56 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 24021-1-AP

Synonyme

CXorf56, STEEP, STING ER exit protein, UPF0428 protein CXorf56

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  WB analysis of HeLa using 24021-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24021-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24021-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24021-1-AP (CXorf56 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 24021-1-AP

  IP result of anti-CXorf56 (IP:24021-1-AP, 4ug; Detection:24021-1-AP 1:1000) with mouse kidney tissue lysate 8000ug.

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  IF Staining of HeLa using 24021-1-AP

  Immunofluorescent analysis of HeLa cells using 24021-1-AP (CXorf56 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IP: See 1 publications below

Produktinformation

24021-1-AP bindet in WB, IF/ICC, IP, ELISA CXorf56 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXorf56 fusion protein Ag19856
• Vollständiger Name: chromosome X open reading frame 56
• Berechnetes Molekulargewicht: 222 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC023506
• Gene symbol: CXorf56
• Gene ID (NCBI): 63932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CxORF56 (also known as STEEP) is an evolutionarily highly conserved protein whose function is unknown. Recently CxORF56 has been identified as a protein that interacts with STING, and it is essential for STING ER exit. CxORF56-dependent STING ER exit is essential for STING signaling in antiviral defense and in inflammatory diseases.

Publikationen

Species	Application	Title
human	WB	EBioMedicine; A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology.; Authors - Thaneas Prabakaran
monkey	WB	J Virol; The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1; Authors - Feifei Diao
human	WB,IP	NPJ Breast Cancer; Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer; Authors - Ying Zhu
human	WB	Nat Commun; Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds; Authors - Bao-Cun Zhang