CYC1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYC1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

30

Rezensionen (1)

Kat-Nr. : 10242-1-AP

Synonyme

Complex III subunit 4, Complex III subunit IV, CYC1, cytochrome c 1, UQCR4

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Galerie der Validierungsdaten

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  WB analysis of human brain using 10242-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10242-1-AP (CYC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10242-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10242-1-AP (CYC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10242-1-AP (CYC1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10242-1-AP (CYC1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 10242-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10242-1-AP (CYC1 antibody) at dilution of 1:50 (under 25x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 29 publications below
• IHC: See 2 publications below

Produktinformation

10242-1-AP bindet in WB, IHC, ELISA CYC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYC1 fusion protein Ag0291
• Vollständiger Name: cytochrome c-1
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC001006
• Gene symbol: CYC1
• Gene ID (NCBI): 1537
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYC1, also named as Cytochrome c-1, belongs to the cytochrome c family. Cytochrome c1 is one respiratory subunit of the 11 subunits of the cytochrome bc1 complex of the mitochondrial electron-transfer chain. It mediates the transfer of an electron from Rieske iron-sulfur protein to cytochrome c(PMID:3036122 ). CYC1 mediates apoptosis. In this pathway, a variety of apoptotic stimuli cause CYC1 release from mitochondria, which in turn induces a series of biochemical reactions that result incaspase activation and subsequent cell death.(PMID:15189137) While CYC1 release and DNA fragmentation are unaffected by the noncleavablep75 mutant, mitochondrial morphology of dying cells is maintained, and loss of plasma membrane integrity is delayed(PMID:15186778). The protein migrated as a 35 kd band in SDS page.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	EMBO J; Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV.; Authors - Margherita Protasoni
human,mouse	WB	Cell Res; Caspase cleavage of cytochrome c1 disrupts mitochondrial function and enhances cytochrome c release.; Authors - Zhu Yushan Y
human	WB	Elife; Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.; Authors - Maxence Le Vasseur
mouse	WB	Redox Biol; Examination of bioenergetic function in the inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice.; Authors - Manish S Bharadwaj
human	WB	Environ Pollut; Mitochondrial respiratory chain dysfunction mediated by ROS is a primary point of fluoride-induced damage in Hepa1-6 cells.; Authors - Hong-Wei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (09-16-2019)

The nitrocellulose membrane was blocked with PBS, 5% BSA, w/ Tween-20, yet high levels of background was observed.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, normoxic (lane1), hypoxic (lane2)

CYC1 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, normoxic (lane1), hypoxic (lane2) (Cat no:10242-1-AP)