Cytoglobin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cytoglobin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 13317-1-AP

Synonyme

CYGB, EC:1.11.1.-, EC:1.14.12.-, EC:1.15.1.1, EC:1.7.-.-

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  WB analysis of mouse heart using 13317-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 13317-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 13317-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 13317-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13317-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 13317-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 13317-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13317-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13317-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 13317-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human uterus using 13317-1-AP

  human uterus tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13317-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13317-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13317-1-AP (Cytoglobin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13317-1-AP (Cytoglobin antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13317-1-AP (Cytoglobin antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human stomach using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13317-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13317-1-AP (Cytoglobin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13317-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cytoglobin antibody (13317-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 13317-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Cytoglobin antibody (13317-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, humanes Herzgewebe, humanes Uterusgewebe, Maushirngewebe, Mausnierengewebe, Mauslungengewebe, Maus-Dünndarmgewebe, Mausmilzgewebe, Maus-Magengewebe, PC-3-Zellen, Rattennierengewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Prostatakarzinomgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13317-1-AP bindet in WB, IHC, IF/ICC, ELISA Cytoglobin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cytoglobin fusion protein Ag4161
• Vollständiger Name: cytoglobin
• Berechnetes Molekulargewicht: 190 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21-29 kDa
• GenBank-Zugangsnummer: BC029798
• Gene symbol: Cytoglobin
• Gene ID (NCBI): 114757
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytoglobin, also named as Histoglobin, HGb, STAP and CYGB, belongs to the globin family. Cytoglobin may have a protective function during conditions of oxidative stress. It may be involved in intracellular oxygen storage or transfer. It serves as a defensive mechanism against oxidative stress both in vitro and in vivo.(PMID:21224051) Cytoglobin is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues. The protein, with calculated MW of 21 kd, runs as a 29kd one in canine retina, kidney, liver, lung, and heart. It may be caused by posttranslational modification of the protein. Various immuno-staining patterns were reported including nuclear, cytoplasm and extracellular location.

Publikationen

Species	Application	Title
rat,mouse,human	WB,IF	Arterioscler Thromb Vasc Biol; The Hemoglobin Homolog Cytoglobin in Smooth Muscle Inhibits Apoptosis and Regulates Vascular Remodeling.; Authors - Frances L Jourd'heuil ; KD Validated
human	WB	Antioxid Redox Signal; The anti-oxidative role of cytoglobin in podocytes: implications for a role in chronic kidney disease.; Authors - Elisa B Randi
human	WB	Antioxidants (Basel); Cytoglobin Silencing Promotes Melanoma Malignancy but Sensitizes for Ferroptosis and Pyroptosis Therapy Response; Authors - Joey De Backer ; KD Validated
mouse	IHC	J Cell Physiol; Integrative proteomics and immunochemistry analysis of the factors in the necrosis and repair in acetaminophen-induced acute liver injury in mice.; Authors - Qin Feng
human	WB	Mol Med Rep; Intranasal administration of Cytoglobin modifies human umbilical cord‑derived mesenchymal stem cells and improves hypoxic‑ischemia brain damage in neonatal rats by modulating p38 MAPK signaling‑mediated apoptosis.; Authors - Hanhua Yang
human	WB	Vascul Pharmacol; Endothelial cell-induced cytoglobin expression in vascular smooth muscle cells contributes to modulation of nitric oxide.; Authors - Brenda Lilly