CYLD Monoklonaler Antikörper

CYLD Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G2F4

Kat-Nr. : 66858-1-Ig

Synonyme

CDMT, CYLD, CYLD1, CYLDI, Deubiquitinating enzyme CYLD, EAC, FLJ20180, FLJ31664, FLJ78684, HSPC057, KIAA0849, MFT, MFT1, SBS, TEM, Ubiquitin thiolesterase CYLD, USPL2

---

Galerie der Validierungsdaten

• 

  WB analysis using 66858-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

• 

  WB analysis using 66858-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66858-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66858-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66858-1-Ig (CYLD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of SH-SY5Y using 66858-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of SH-SY5Y using 66858-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CYLD antibody (66858-1-Ig, Clone: 1G2F4 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, A431-Zellen, HEK-293-Zellen, Kaninchenhirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66858-1-Ig bindet in WB, IF, ELISA CYLD und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYLD fusion protein Ag28333
• Vollständiger Name: cylindromatosis (turban tumor syndrome)
• Berechnetes Molekulargewicht: 107 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC012342
• Gene symbol: CYLD
• Gene ID (NCBI): 1540
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYLD, also named as CYLD1, belongs to the peptidase C67 family. It is the protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. CYLD has endodeubiquitinase activity and plays an important role in the regulation of pathways leading to NF-kappa-B activation. CYLD contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. It is a negative regulator of Wnt signaling. CYLD inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. CYLD plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. It is required for normal cell cycle progress and normal cytokinesis. CYLD inhibits nuclear translocation of NF-kappa-B and plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. It is dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. CYLD negatively regulates TNFRSF11A signaling and osteoclastogenesis.