CYP11A1 Polyklonaler Antikörper

CYP11A1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(67)

Kat-Nr. : 13363-1-AP

Synonyme

Cholesterol desmolase, Cholesterol side-chain cleavage enzyme, mitochondrial, CYP11A, CYPXIA1, Cytochrome P450 11A1

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  WB analysis using 13363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13363-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13363-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP11A1 antibody (13363-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, HeLa-Zellen, Maus-Eierstockgewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 59 publications below
• IHC: See 5 publications below
• IF: See 9 publications below

Produktinformation

13363-1-AP bindet in WB, IHC, IF/ICC, ELISA CYP11A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP11A1 fusion protein Ag4182
• Vollständiger Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 521 aa, 60 kDa
• Beobachtetes Molekulargewicht: 49-52 kDa
• GenBank-Zugangsnummer: BC032329
• Gene symbol: CYP11A1
• Gene ID (NCBI): 1583
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP11A1 is also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. It is the first enzyme of all steroidogenic pathways, its elimination results in syndromes of steroid deficiency, including electrolyte imbalance, aberrant gene regulation, and suppression of male sex organ development(PMID: 12145347). Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al. The murine 32-kDa isoform of Cyp11a1 is not produced by omission of the first ATG-containing exon (as is the case for human CYP11A1) but perhaps by alternative translational initiation(PMID:19342447).

Publikationen

Species	Application	Title
rat	WB	Science; Pregnenolone can protect the brain from cannabis intoxication.; Authors - Monique Vallée
mouse	WB	Brain Behav Immun; Placenta-specific CYP11A1 overexpression lead to autism-like symptom in offspring with altered steroid hormone biosynthesis in the placenta-brain axis and rescued by vitamin D intervention; Authors - Heng Yin
human	WB	Nat Commun; 1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.; Authors - Michaela Schedel
mouse	WB	Autophagy; Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.; Authors - Wenwen Liu
mouse	WB	Environ Pollut; Integrated RNA sequencing and biochemical studies reveal endoplasmic reticulum stress and autophagy dysregulation contribute to Tri (2-Ethylhexyl) phosphate (TEHP)-induced cell injury in Sertoli cells; Authors - Pengchen Chen
human	WB,IHC	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou