CYP1A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP1A1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(105)

Kat-Nr. : 13241-1-AP

Synonyme

AHH, AHRR, CP11, CYP1, CYPIA1

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  WB analysis of mouse cerebellum using 13241-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13241-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13241-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13241-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13241-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13241-1-AP (CYP1A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 13241-1-AP

  IP result of anti-CYP1A1 (IP:13241-1-AP, 4ug; Detection:13241-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 13241-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13241-1-AP (CYP1A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 13241-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP1A1 antibody (13241-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 13241-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CYP1A1 Polyclonal antibody (13241-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HepG2-Zellen, Maus-Cerebellum-Gewebe, Mauslungengewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 89 publications below
• IHC: See 13 publications below
• IF: See 13 publications below

Produktinformation

13241-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA CYP1A1 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP1A1 fusion protein Ag3990
• Vollständiger Name: cytochrome P450, family 1, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 512 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50-58 kDa
• GenBank-Zugangsnummer: BC023019
• Gene symbol: CYP1A1
• Gene ID (NCBI): 1543
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Environ Int; Novel fluorescent and secreted transcriptional reporters for quantifying activity of the xenobiotic sensor aryl hydrocarbon receptor (AHR); Authors - Séverine A Degrelle
mouse,human	WB	Free Radic Biol Med; 5-Methoxyflavone ameliorates non-alcoholic fatty liver disease through targeting the cytochrome P450 1A1; Authors - Yurou Zhang
mouse,human	WB	Phytother Res; Quercetin ameliorates ulcerative colitis by activating aryl hydrocarbon receptor to improve intestinal barrier integrity; Authors - Xiaojing Wang
mouse	IF	NPJ Regen Med; Myeloid cell-associated aromatic amino acid metabolism facilitates CNS myelin regeneration; Authors - Jingwen Hu
		Cell Rep; Hypoxia-induced PRMT1 methylates HIF2β to promote breast tumorigenesis via enhancing glycolytic gene transcription; Authors - Wen-Juan Li
human	WB	Environ Pollut; Regioselective hydroxylation of carbendazim by mammalian cytochrome P450: A combined experimental and computational study.; Authors - Xia Lv