CYP1A2-Specific Polyklonaler Antikörper

CYP1A2-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Kat-Nr. : 19936-1-AP

Synonyme

CYP1A2, Cholesterol 25-hydroxylase, CP12, CP1A2, CYPIA2

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  WB analysis of mouse liver using 19936-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19936-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 19936-1-AP

  IP result of anti-CYP1A2-Specific (IP:19936-1-AP, 4ug; Detection:19936-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 19936-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19936-1-AP (CYP1A2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse liver using 19936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19936-1-AP (CYP1A2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19936-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP1A2-Specific antibody (19936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 19936-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CYP1A2-Specific Polyclonal antibody (19936-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 39 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Produktinformation

19936-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA CYP1A2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 1, subfamily A, polypeptide 2
• Berechnetes Molekulargewicht: 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: NM_000761
• Gene symbol: CYP1A2
• Gene ID (NCBI): 1544
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP1A2, also named as CP12; P3-450 and P450(PA), belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP1A2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active of CYP1A2 is in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. It also acts in the metabolism of aflatoxin B1 and acetaminophen. CYP1A2 participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. It catalizes the reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O. The antibody is specific to CYP1A2.

Publikationen

Species	Application	Title
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
mouse	IF	Hepatology; Self-renewal of hepatoblasts under chemically defined conditions by reiterative growth factor and chemical screening.; Authors - Linjie Lv
human	IF	Acta Biomater; Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.; Authors - Kun Du
human	IF	ACS Appl Mater Interfaces; Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip; Authors - Xiaofang Du
mouse	WB	Br J Pharmacol; Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.; Authors - Li-Ping Jiang
mouse	WB,IF	Cell Mol Gastroenterol Hepatol; Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice.; Authors - Fenja A Schuran