CYP1B1 Monoklonaler Antikörper

CYP1B1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1G8D4

Kat-Nr. : 67033-1-Ig

Synonyme

GLC3A, EC:4.2.1.152, EC:1.14.14.1, CYPIB1, CP1B

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  WB analysis using 67033-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 67033-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67033-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67033-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67033-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67033-1-Ig (CYP1B1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67033-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 67033-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 67033-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67033-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human rectal cancer using 67033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human rectal cancer using 67033-1-Ig

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67033-1-Ig (CYP1B1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, PC-3-Zellen, Hausschwein-Hirngewebe, Rattenhirngewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67033-1-Ig bindet in WB, IHC, ELISA CYP1B1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP1B1 fusion protein Ag13380
• Vollständiger Name: cytochrome P450, family 1, subfamily B, polypeptide 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC012049
• Gene symbol: CYP1B1
• Gene ID (NCBI): 1545
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cytochrome P450 CYP1B1 is a recently cloned dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes. CYP1B1 was found to be expressed at a high frequency in a wide range of human cancers of different histogenetic types, including cancers of the breast, colon, lung, esophagus, skin, lymph node, brain, and testis. There was no detectable immunostaining for CYP1B1 in normal tissues.(PMID:9230218). CYP1B1 is a 60 kDa protein that it also can be detected a band of 51 kDa or 57 kDa through the western blot(PMID:9525272; PMID: 9525272).

Publikationen

Species	Application	Title
human,mouse	WB	Placenta; Indole-3-lactic acid derived from tryptophan metabolism promotes trophoblast migration and invasion by activating the AhR/VCAN pathway; Authors - Yingying Wei