CYP20A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP20A1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16702-1-AP

Synonyme

CYP M, CYP20A1, Cytochrome P450 20A1

---

Galerie der Validierungsdaten

• 

  WB analysis using 16702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16702-1-AP

  WB result of CYP20A1 antibody (16702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP20A1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 16702-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16702-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16702-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16702-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 16702-1-AP

  IP result of anti-CYP20A1 (IP:16702-1-AP, 4ug; Detection:16702-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

• 

  IHC staining of human liver cancer using 16702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, K-562-Zellen, Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16702-1-AP bindet in WB, IP, IHC, ELISA CYP20A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP20A1 fusion protein Ag10099
• Vollständiger Name: cytochrome P450, family 20, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 462 aa, 52 kDa
• Beobachtetes Molekulargewicht: 45-52 kDa
• GenBank-Zugangsnummer: BC020616
• Gene symbol: CYP20A1
• Gene ID (NCBI): 57404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.