CYP20A1 Monoklonaler Antikörper

CYP20A1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A9E2

Kat-Nr. : 67433-1-Ig

Synonyme

2A9E2, CYP M, Cytochrome P450 20A1, EC:1.14.-.-, UNQ667/PRO1301

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  WB analysis using 67433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67433-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67433-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67433-1-Ig bindet in WB, IHC, IF/ICC, ELISA CYP20A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP20A1 fusion protein Ag9715
• Vollständiger Name: cytochrome P450, family 20, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 462 aa, 52 kDa
• Beobachtetes Molekulargewicht: 49-52 kDa
• GenBank-Zugangsnummer: BC020616
• Gene symbol: CYP20A1
• Gene ID (NCBI): 57404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.