CYP27A1 Monoklonaler Antikörper

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• KD/KO Validated

CYP27A1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

3G3F7

Kat-Nr. : 67045-1-Ig

Synonyme

3G3F7, 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase, CP27, CTX, CYP27

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  WB analysis of Caco-2 using 67045-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67045-1-Ig

  WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells.

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  WB analysis of rat liver using 67045-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67045-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67045-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67045-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67045-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67045-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human liver cancer using 67045-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HepG2-Zellen, HT-29-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

67045-1-Ig bindet in WB, IHC, IF-P, ELISA CYP27A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CYP27A1 fusion protein Ag7746
• Vollständiger Name: cytochrome P450, family 27, subfamily A, polypeptide 1
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 54-60 kDa
• GenBank-Zugangsnummer: BC051851
• Gene symbol: CYP27A1
• Gene ID (NCBI): 1593
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Bone Miner Res; CYP4A22 Loss-of-Function Causes A New Type of Vitamin D-dependent Rickets (VDDR1C); Authors - Xiaohong Duan
mouse	WB	J Ethnopharmacol; Penthorum chinense Pursh. extract attenuates non-alcholic fatty liver disease by regulating gut microbiota and bile acid metabolism in mice.; Authors - Xiaoxi Li
mouse	WB	Nat Microbiol; Akkermansia muciniphila protects mice against an emerging tick-borne viral pathogen; Authors - Jinyan Xie
human	WB	Front Immunol; Construction of a lipid metabolism-related risk model for hepatocellular carcinoma by single cell and machine learning analysis; Authors - Lisha Mou
mouse	WB	Adv Sci (Weinh); Geniposidic Acid Targeting FXR "S332 and H447" Mediated Conformational Change to Upregulate CYPs and miR-19a-3p to Ameliorate Drug-Induced Liver Injury; Authors - Minqi Fan
rat	WB,IF	J Ethnopharmacol; Integration of network pharmacology, UHPLC-Q exactive orbitrap HRMS technique and metabolomics to elucidate the active ingredients and mechanisms of compound danshen pills in treating hypercholesterolemic rats; Authors - Shanlan Li