CYP2E1-Specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CYP2E1-Specific Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

87

Rezensionen (2)

Kat-Nr. : 19937-1-AP

Synonyme

4 nitrophenol 2 hydroxylase, CPE1, CYP2E, CYP2E1, CYP-2E1, CYP2E1-Specific, CYPIIE1, Cytochrome P450 2E1, Cytochrome P450 J, P450 J, P450C2E

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Galerie der Validierungsdaten

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  WB analysis using 19937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19937-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 19937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19937-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19937-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 19937-1-AP

  Immunofluorescent analysis of Hela cells, using CYP2E1 antibody 19937-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Jurkat-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 80 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Produktinformation

19937-1-AP bindet in WB, IHC, IF, ELISA CYP2E1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytochrome P450, family 2, subfamily E, polypeptide 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: NM_000773
• Gene symbol: CYP2E1
• Gene ID (NCBI): 1571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP2E1, also named as CYPIIE1, 4-nitrophenol 2-hydroxylase, P450-J and CYP2E, belongs to the cytochrome P450 family. CYP2E1 metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. It inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. CYP2E1 catalyze the reaction: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O. CYP2E1 can be detected as 50-57 kDa(full-length) and ~40 kDa(N-terminal signal peptide cleaved) (PMID: 21130154). The antibody is specific to CYP2E1.

Publikationen

Species	Application	Title
mouse	WB	Cell Host Microbe; Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice; Authors - Yunong Zeng
mouse	WB	Cell Metab; Fatty Liver Disease Caused by High-Alcohol-Producing Klebsiella pneumoniae.; Authors - Jing Yuan
mouse	WB	Nat Commun; The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury; Authors - Hakjoo Lee
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	Cell Biol Toxicol; Protecting mitochondria via inhibiting VDAC1 oligomerization alleviates ferroptosis in acetaminophen-induced acute liver injury.; Authors - Baolin Niu
mouse	WB	Cell Death Dis; Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.; Authors - Cheng Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with Cyp2e1 antibody at dilution of 1:5000 incubated at 4 degree overnight. Bands appeared at about 55 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse liver tissue

FH

James (Verified Customer) (11-18-2022)

Detection of CYP2E1 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

CYP2E1-Specific Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:19937-1-AP)