CYP4A11 Polyklonaler Antikörper

CYP4A11 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11688-1-AP

Synonyme

20 HETE synthase, CP4Y, CYP4A11, CYP4A2, CYP4AII, CYPIVA11, Cytochrome P 450HK omega, Cytochrome P450 4A11, Cytochrome P450HL omega, Fatty acid omega hydroxylase, Lauric acid omega hydroxylase

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11688-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 11688-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11688-1-AP (CYP4A11 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11688-1-AP (CYP4A11 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11688-1-AP (CYP4A11 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:300-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

11688-1-AP bindet in WB, IHC, ELISA CYP4A11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CYP4A11 fusion protein Ag2300
• Vollständiger Name: cytochrome P450, family 4, subfamily A, polypeptide 11
• Berechnetes Molekulargewicht: 519 aa, 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC022851
• Gene symbol: CYP4A11
• Gene ID (NCBI): 1579
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Free Radic Biol Med; PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase.; Authors - Xue Chen
rat	WB	Food Funct; Correction: Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
rat	WB	Food Funct; Total saponins from Rosa laevigata Michx fruit attenuates hepatic steatosis induced by high-fat diet in rats.; Authors - Deshi Dong
human	WB	Forensic Sci Int; Identification of the miRNA-3185/CYP4A11 axis in cardiac tissue as a biomarker for mechanical asphyxia.; Authors - Liujun Han
mouse,rat	WB	Chem Biol Interact; Activation of 20-HETE/PPARs involved in reno-therapeutic effect of naringenin on diabetic nephropathy.; Authors - Shumei Ding