CYP7B1 Rekombinanter Antikörper

CYP7B1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230160H5

Kat-Nr. : 82927-1-RR

Synonyme

CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase, 24-hydroxycholesterol 7-alpha-hydroxylase

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  WB analysis using 82927-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82927-1-RR (CYP7B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82927-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HEK-293 using 82927-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (82927-1-RR, Clone: 230160H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HeLa using 82927-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CYP7B1 Recombinant antibody (82927-1-RR, Clone:230160H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82927-1-RR bindet in WB, IF/ICC, FC (Intra), ELISA CYP7B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CYP7B1 fusion protein Ag19374
• Vollständiger Name: cytochrome P450, family 7, subfamily B, polypeptide 1
• Berechnetes Molekulargewicht: 506 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC136574
• Gene symbol: CYP7B1
• Gene ID (NCBI): 9420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.