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Calnexin Monoklonaler Antikörper

Calnexin Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(82)

CloneNo.

2A2C6

Kat-Nr. : 66903-1-Ig

Synonyme

CANX, 2A2C6, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
Erfolgreiche Detektion in IHChumanes Leberzirrhosegewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:20000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66903-1-Ig bindet in WB, IHC, IF, ELISA Calnexin und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Hausschwein, Kaninchen, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Calnexin fusion protein Ag25535
Vollständiger Name calnexin
Berechnetes Molekulargewicht 90 kDa
Beobachtetes Molekulargewicht 90 kDa
GenBank-ZugangsnummerBC003552
Gene symbol Calnexin
Gene ID (NCBI) 821
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Calnexin antibody 66903-1-IgProtokoll herunterladen
IHC protocol for Calnexin antibody 66903-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Cell Res

Targeting ATAD3A-PINK1-mitophagy axis overcomes chemoimmunotherapy resistance by redirecting PD-L1 to mitochondria

Authors - Xiao-Qing Xie
mouse,humanWB,IF

Nat Commun

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

Authors - Xiangling Xiao
humanWB

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
mouseIF

Acta Pharm Sin B

The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.

Authors - Weimin Yu
ratWB

J Control Release

Extracellular vesicles encapsulated with caspase-1 inhibitor ameliorate experimental autoimmune myasthenia gravis through targeting macrophages

Authors - Yang Zhou
mouseIF

Clin Transl Med

Targeting KDM4 family epigenetically triggers antitumour immunity via enhancing tumour-intrinsic innate sensing and immunogenicity

Authors - Mayu Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Samantha (Verified Customer) (03-18-2024)

Tested on HeLA cells and produced clean bands that were cut out and analysed with Mass Spectrometry

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/10,000
  • Cell Tissue Type: HeLa Cells
FH

Amy (Verified Customer) (11-08-2023)

Clean antibody with strong band for WB in HEK293T lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: HEK293T