Calsequestrin 1 Polyklonaler Antikörper

Calsequestrin 1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 26665-1-AP

Synonyme

Calmitin, Calsequestrin 1, CASQ, CASQ1, PDIB1

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• 

  WB analysis of mouse heart using 26665-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26665-1-AP (Calsequestrin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle tissue using 26665-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26665-1-AP( Calsequestrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle tissue using 26665-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 26665-1-AP( Calsequestrin antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

26665-1-AP bindet in WB, IHC, ELISA Calsequestrin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Calsequestrin 1 fusion protein Ag24684
• Vollständiger Name: calsequestrin 1 (fast-twitch, skeletal muscle)
• Berechnetes Molekulargewicht: 390 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa, 63 kDa
• GenBank-Zugangsnummer: BC022289
• Gene symbol: Calsequestrin 1
• Gene ID (NCBI): 844
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Front Mol Biosci; Altered Glycosylation in the Aging Heart.; Authors - Patricia Franzka
human	IHC	J Neuropathol Exp Neurol; Morphological Alterations of the Sarcotubular System in Permanent Myopathy of Hereditary Hypokalemic Periodic Paralysis with a Mutation in the CACNA1S Gene.; Authors - Takamura Nagasaka
mouse	WB	J Muscle Res Cell Motil; Differential regulation of Actn2 and Actn3 expression during unfolded protein response in C2C12 myotubes.; Authors - Nagakatsu Harada
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi
mouse	WB	Sci Rep; ERG1A K+ channel increases intracellular calcium concentration through modulation of calsequestrin1 in C2C12 myotubes; Authors - Gregory H Hockerman

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marco (Verified Customer) (07-19-2024)

Took the risk and tried it in neonatal rat cardiomyocytes. It worked !

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC, NRVCM

Calsequestrin 1 Antibody Western Blot validation (1:1000 dilution) in iPSC, NRVCM (Cat no:26665-1-AP)