Caspase 9/P35 Monoklonaler Antikörper

Caspase 9/P35 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(123)

CloneNo.

1B7G2

Rezensionen (2)

Kat-Nr. : 66169-1-Ig

Synonyme

CASP9, Caspase 9, Caspase 9/p35/p10, 1B7G2, APAF 3

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  WB analysis of Jurkat using 66169-1-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/P35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66169-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/P35 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66169-1-Ig

  IP result of anti-Caspase 9/P35 (IP:66169-1-Ig, 5ug; Detection:66169-1-Ig 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human lymphoma using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/P35 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/P35 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66169-1-Ig (Caspase 9/P35 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 66169-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66169-1-Ig (Caspase 9/P35 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 66169-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66169-1-Ig(Caspase 9/P35 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 120 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• ELISA: See 1 publications below

Produktinformation

66169-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA Caspase 9/P35 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caspase 9/P35 fusion protein Ag20813
• Vollständiger Name: caspase 9, apoptosis-related cysteine peptidase
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa, 35 kDa
• GenBank-Zugangsnummer: BC002452
• Gene symbol: Caspase 9
• Gene ID (NCBI): 842
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].

Publikationen

Species	Application	Title
human	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
mouse	WB	J Pineal Res; Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.; Authors - Wenkai Ren
human	WB	Redox Biol; Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.; Authors - Zhongren Xu
human	WB,IF	Chem Sci; Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.; Authors - Meng Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-29-2025)

Great antibody for human Caspase9

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: RKO

Caspase 9/P35 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:66169-1-Ig)

FH

Tsimafei (Verified Customer) (08-07-2024)

Antibody detect a band of the correct size

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

Caspase 9/P35 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:66169-1-Ig)