Catalase Monoklonaler Antikörper

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• KD/KO Validated

Catalase Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

CloneNo.

1F8D7

Kat-Nr. : 66765-1-Ig

Synonyme

CAT, catalase, Cell and organelle markers, Peroxisome Marker

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Galerie der Validierungsdaten

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  WB analysis using 66765-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HeLa using 66765-1-Ig

  WB result of Catalase antibody (66765-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells.

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  WB analysis using 66765-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66765-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66765-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66765-1-Ig (Catalase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 66765-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 66765-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66765-1-Ig (Catalase antibody) at dilution of 1:500 (under 40x lens).

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  IF Staining of HepG2 using 66765-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Catalase antibody (66765-1-Ig, Clone: 1F8D7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HepG2 using 66765-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66765-1-Ig (Catalase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:100-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 3 publications below

Produktinformation

66765-1-Ig bindet in WB, IHC, IF, ELISA Catalase und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Ziege
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Catalase fusion protein Ag16916
• Vollständiger Name: catalase
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC112219
• Gene symbol: CAT
• Gene ID (NCBI): 847
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

Publikationen

Species	Application	Title
pig	WB	Microbiome; Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.; Authors - Leli Wang
mouse	WB	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
human	IF	Elife; Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress; Authors - Jinyang Wang
goat	WB	Oxid Med Cell Longev; Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.; Authors - Tian-Yu Feng
rat	WB	Oxid Med Cell Longev; Cryptotanshinone Protects against PCOS-Induced Damage of Ovarian Tissue via Regulating Oxidative Stress, Mitochondrial Membrane Potential, Inflammation, and Apoptosis via Regulating Ferroptosis.; Authors - Honglin Liu
mouse	WB	Antioxidants (Basel); High-Intensity Interval Training and Moderate-Intensity Continuous Training Attenuate Oxidative Damage and Promote Myokine Response in the Skeletal Muscle of ApoE KO Mice on High-Fat Diet.; Authors - Linjia Wang