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Cathepsin L Polyklonaler Antikörper

Cathepsin L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 27952-1-AP

Synonyme

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen
Erfolgreiche Detektion in IHChumanes Leberkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27952-1-AP bindet in WB, IHC, IF, ELISA Cathepsin L und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Cathepsin L fusion protein Ag27813
Vollständiger Name cathepsin L1
Berechnetes Molekulargewicht 38 kDa
Beobachtetes Molekulargewicht 36-39 kDa, 29 kDa
GenBank-ZugangsnummerBC012612
Gene symbol Cathepsin L
Gene ID (NCBI) 1514
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Cathepsin L antibody 27952-1-APProtokoll herunterladen
IHC protocol for Cathepsin L antibody 27952-1-APProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Front Pharmacol

Nodosin Exerts an Anti-Colorectal Cancer Effect by Inhibiting Proliferation and Triggering Complex Cell Death in Vitro and in Vivo

Authors - Huixia Fan
humanWB

Acta Pharmacol Sin

Salvianolic acid B suppresses hepatic fibrosis by inhibiting ceramide glucosyltransferase in hepatic stellate cells

Authors - Zi-Bo Li
humanIHC

Transl Oncol

Mitochondria-related chemoradiotherapy resistance genes-based machine learning model associated with immune cell infiltration on the prognosis of esophageal cancer and its value in pan-cancer

Authors - Ziyu Liu
mouseWB

J Med Chem

Design, Synthesis, and Biological Evaluation of 2-Arylaminopyrimidine Derivatives as Dual Cathepsin L and JAK Inhibitors for the Treatment of Acute Lung Injury

Authors - Chunwei Shen
humanWB

Pharmacol Res

The molecular receptor NKBB enhances the persistence and anti-hepatocellular carcinoma activity of GPC3 CAR-T cells

Authors - Minghao Sui
humanWB,IHC,IF

World J Gastroenterol

Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer

Authors - Lu-Xi Xiao
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