Caveolin-1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Caveolin-1 Monoklonal Antikörper für IF-P, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

6C2B2

Kat-Nr. : CL488-66067

Synonyme

Caveolin 1, CAV1, CGL3, CAV, BSCL3

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  IF Staining of human skin cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 Caveolin-1 antibody (CL488-66067, Clone: 6C2B2 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  IF Staining of human liver cancer using CL488-66067

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CL488-66067 (Caveolin-1 antibody) at dilution of 1:100.

• 

  FC experiment of HeLa using CL488-66067

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Caveolin-1 (CL488-66067, Clone:6C2B2) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe, humanes Hautkrebsgewebe
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-66067 bindet in IF-P, FC (Intra) Caveolin-1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Caveolin-1 fusion protein Ag8049
• Vollständiger Name: caveolin 1, caveolae protein, 22kDa
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC006432
• Gene symbol: CAV1
• Gene ID (NCBI): 857
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in gastric Cancer (PMID: 25339030).

Publikationen

Species	Application	Title
	IF	Nature; Glycosphingolipid synthesis mediates immune evasion in KRAS-driven cancer; Authors - Mariluz Soula